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Study Confirms Synergistic Antimicrobial Effect of Natural Essential Oil Constituents

Here’s wonderful evidence that nature is making balanced medicine ~ that the past ventures of western science to “isolate the active ingredient” in a natural substance, then use ONLY that is really not necessary. In fact, it’s been somewhat wasteful in term of the energy requirements needed to single out the “active ingredient” rather than [...]

Here’s wonderful evidence that nature is making balanced medicine ~ that the past ventures of western science to “isolate the active ingredient” in a natural substance, then use ONLY that is really not necessary. In fact, it’s been somewhat wasteful in term of the energy requirements needed to single out the “active ingredient” rather than just use the whole natural substance itself…perhaps we’re a litte biased here at Ananda ūüôā …knowing that whole, true essential oils are medicines, and made-made isolates rarely have the same wholistic therapeutic action.

In this study performed at Institute of Complementary Medicine in Zurich, Switzerland, the antimicrobial effects of the individual chemical constituents of Thyme essential oil were studied each individually, then together. It turns out their effects are additive, and the conclusion is that a natural balance of several active ingredients may have the greatest efficacy.

Study: Additive Antmicrobial Effects of the Active Components of the Essential Oil of Thymus vulgaris – Chemotype Carvacrol.

Iten F, Saller R, Abel G, Reichling J. at the Institute of Complementary Medicine, University Hospital Zurich, Zurich, Switzerland.

Herbal remedies are multicomponent mixtures by their nature as well as by pharmaceutical definition. Being a multicomponent mixture is not only a crucial property of herbal remedies, it also represents a precondition for interactions such as synergism or antagonism. Until now, only a few phytomedicines are accurately described concerning the interactions of their active components. The aim of this study was to search for interactions within such a naturally given multi-component mixture and to discuss the pharmaceutical and clinical impacts. The thyme oil chosen for the examination belongs to the essential oils with the most pronounced antimicrobial activity. Antibiotic activity of thyme oil and single active components were tested against six different strains of microorganisms. The checkerboard assay was used to search for interactions. The time-kill assay was used to verify the observed effects and to get information about the temporal resolution of the antimicrobial activity. The degree of the detected interactions corresponded with the demarcating FICI measure of 0.5, which separates the additive from the over-additive (synergistic) effects. Therefore, the observed effect was called a “borderline case of synergism” or, respectively, “partial synergism”. Partial synergism was observed only in the presence of KLEBSIELLA PNEUMONIAE. Additive antimicrobial activity was observed for the combination of the two monosubstances carvacrol plus linalool and thymol plus linalool as well as with the combination of the two essential oils of the carvacrol and linalool chemotypes. An increase of the carvacrol oil concentration from one to two times the MIC resulted in a considerable acceleration of the kill-rate. Thyme oil is composed of several different components that show antimicrobial activity (at least: carvacrol, thymol and linalool). The antimicrobial activity of thyme oil is partly based on additive effects, which might especially enhance the rapidity of the antimicrobial action. In addition, a mixture of several active ingredients that varies in its composition from year to year and from lot to lot as is the case with herbal remedies may be more stable concerning the antimicrobial activity than mixtures containing just a single active component.

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